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5-Aza-CdR Regulates RASSF1A By Inhibiting DNMT1 To Affect Colon Cancer Cell Proliferation, Migration And Apoptosis

Authors Chen J, Wu L, Xu H, Cheng S

Received 3 September 2019

Accepted for publication 22 October 2019

Published 8 November 2019 Volume 2019:11 Pages 9517—9528

DOI https://doi.org/10.2147/CMAR.S229726

Checked for plagiarism Yes

Review by Single-blind

Peer reviewers approved by Dr Melinda Thomas

Peer reviewer comments 3

Editor who approved publication: Dr Antonella D'Anneo


Jinyuan Chen,1 Lixiang Wu,2 Hong Xu,3 Shubang Cheng1

1Department of Gastrointestinal Surgery, People’s Hospital of Longhua, Shenzhen, Guangdong 518109, People’s Republic of China; 2Guangzhou Concord Cancer Center, Guangzhou, Guangdong 510620, People’s Republic of China; 3Department of Pharmacy, People’s Hospital of Longhua, Shenzhen, Guangdong 518109, People’s Republic of China

Correspondence: Shubang Cheng
Department of Gastrointestinal Surgery, People’s Hospital of Longhua, Shenzhen, Guangdong 518109, People’s Republic of China
Email 534898952@qq.com

Objective: To evaluate 5-Aza-CdR’s inhibited effects on migration, proliferation, and apoptosis in colon cancer cells and its potential mechanisms.
Methods: HCT-116, SW480, and SW620 were divided into HCT116 group, HCT116+5-Aza-CdR group, SW480 group, SW480+5-Aza-CdR group, SW620 group and SW620+5-Aza according to experimental needs. MTT test was chosen to investigate cell proliferation; Transwell test was used to evaluate cell migration; scratch assay was used to investigate cell invasion; flow cytometry was used to investigate apoptosis; immunofluorescence assay was used to investigate the protein level of DNMT1 and RASSF1A in cells; qRT-PCR was used to examine DNMT1, RASSF1A, RAS, Raf1, MEK, Grb2 and ERK transcription levels.
Results: Compared with HCT116 group, 5-Aza-CdR+HCT116 group inhibited cell proliferation, increased apoptosis rate, decreased invasive ability, decreased DNMT1 expression, increased expression of RASSF1A, decreased expression of RAS, Raf1, MEK, Grb2 and ERK. SW480 was compared with 5-Aza-CdR+SW480 group and SW620 group with 5-Aza-CdR+SW620 group. Their change trend of detection index was similar to that in HCT-116 group and HCT116+5-Aza-CdR group.
Conclusion: 5-Aza-CdR can obviously inhibit the proliferation, migration and invasion of three colon cancer cell lines. Its mechanism maybe relies on the inhibition of DNMT1 mRNA level and protein level and the enhancement of RASSF1A mRNA level and protein level.

Keywords: DNA methylation, colon cancer, RASSF1A, 5-Aza-CdR, DNMT1
 

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