Trazodone effects on [³H]-paroxetine and α₂-adrenoreceptors in platelets of patients with major depression

Original Research

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Authors:

Donatella Marazziti, Giorgio Consoli, Francesca Golia, et al

Published Date May 2010 Volume 2010:6(1) Pages 255 - 259

DOI: http://dx.doi.org/10.2147/NDT.S9279

Donatella Marazziti, Giorgio Consoli, Francesca Golia, Stefano Baroni, Irene Masala, Marina Carlini, Mario Catena Dell’Osso

Dipartimento di Psichiatria, Neurobiologia, Farmacologia e Biotecnologie, University of Pisa, Pisa, Italy

Abstract: Trazodone is an antidepressant which behaves as a selective 5-HT₂ antagonist and 5-HT reuptake inhibitor. The lack of information on its effects in vivo prompted us to evaluate α₂-adrenoceptors by means of the specific binding of [³H]-rauwolscine, and the 5-HT transporter (SERT) by means of the binding of [³H]-paroxetine ([³H]-Par), in platelets of depressed patients, before and after one month of treatment with trazodone (75–300 mg/day). Twenty-five outpatients of both sexes with a diagnosis of major depression, as assessed by the Structured Clinical Interview for DSM IV, were included in the study. Depressive symptoms were evaluated by means of the Hamilton Rating Scale for Depression: the total score (mean ± SD) was 20 ± 6 at baseline (t₀) and 7 ± 4 after one month of treatment (t₁). Platelet membranes, [³H]-rauwolscine and [³H]-Par bindings were carried out according to standardized protocols. The results showed that the B_max values of [³H]-Par were statistically lower at t₁ than at t₀ (733 ± 30 vs 1471 ± 99, P < 0.001), while the K_d and the [³H]-rauwolscine binding parameters remained unchanged. The findings of this study suggest that in vivo trazodone modifies the number of the SERT proteins and that, perhaps, most of its antidepressant properties are related to this activity.

Keywords: trazodone, depression, serotonin, platelets, α₂-adrenoreceptors, [³H]-rauwolscine, serotonin transporter, [³H]-paroxetine