Back to Browse Journals » International Journal of Nanomedicine » Volume 7

Synthesis and application of luminescent single CdS quantum dot encapsulated silica nanoparticles directed for precision optical bioimaging

Authors Veeranarayanan S, Poulose AC, Mohamed MS, Nagaoka Y, Iwai S, Nakagame Y, Kashiwada S, Yoshida Y, Maekawa T, Kumar DS

Published Date July 2012 Volume 2012:7 Pages 3769—3786

DOI http://dx.doi.org/10.2147/IJN.S31310

Received 29 February 2012, Accepted 28 April 2012, Published 17 July 2012

Srivani Veeranarayanan, Aby Cheruvathoor Poulose, M Sheikh Mohamed, Yutaka Nagaoka, Seiki Iwai, Yuya Nakagame, Shosaku Kashiwada, Yasuhiko Yoshida, Toru Maekawa, D Sakthi Kumar

Bio Nano Electronics Research Centre, Graduate School of Interdisciplinary New Science, Toyo University, Kawagoe, Japan

Abstract: This paper presents the synthesis of aqueous cadmium sulfide (CdS) quantum dots (QDs) and silica-encapsulated CdS QDs by reverse microemulsion method and utilized as targeted bio-optical probes. We report the role of CdS as an efficient cell tag with fluorescence on par with previously documented cadmium telluride and cadmium selenide QDs, which have been considered to impart high levels of toxicity. In this study, the toxicity of bare QDs was efficiently quenched by encapsulating them in a biocompatible coat of silica. The toxicity profile and uptake of bare CdS QDs and silica-coated QDs, along with the CD31-labeled, silica-coated CdS QDs on human umbilical vein endothelial cells and glioma cells, were investigated. The effect of size, along with the time-dependent cellular uptake of the nanomaterials, has also been emphasized. Enhanced, high-specificity imaging toward endothelial cell lines in comparison with glioma cells was achieved with CD31 antibody-conjugated nanoparticles. The silica-coated nanomaterials exhibited excellent biocompatibility and greater photostability inside live cells, in addition to possessing an extended shelf life. In vivo biocompatibility and localization study of silica-coated CdS QDs in medaka fish embryos, following direct nanoparticle exposure for 24 hours, authenticated the nanomaterials' high potential for in vivo imaging, augmented with superior biocompatibility. As expected, CdS QD-treated embryos showed 100% mortality, whereas the silica-coated QD-treated embryos stayed viable and healthy throughout and after the experiments, devoid of any deformities. We provide highly cogent and convincing evidence for such silica-coated QDs as a model nanoparticle in practice, to achieve in vitro and in vivo precision targeted imaging.

Keywords: endothelial imaging, CD31, silica nanoparticle, CdS QDs, medaka embryos, biocompatibility

Download Article [PDF] View Full Text [HTML] 

Creative Commons License This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution - Non Commercial (unported, v3.0) License. The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. Permissions beyond the scope of the License are administered by Dove Medical Press Limited. Information on how to request permission may be found at: http://www.dovepress.com/permissions.php

Other articles by this author:

Curcumin and 5-Fluorouracil-loaded, folate- and transferrin-decorated polymeric magnetic nanoformulation: a synergistic cancer therapeutic approach, accelerated by magnetic hyperthermia

Balasubramanian S, Girija AR, Nagaoka Y, Iwai S, Suzuki M,
Kizhikkilot V, Yoshida Y, Maekawa T, Nair SD

International Journal of Nanomedicine 2014, 9:437-459

Published Date: 9 January 2014

Readers of this article also read:

Enhanced absorption of hydroxysafflor yellow A using a self-double-emulsifying drug delivery system: in vitro and in vivo studies

Lv LZ, Tong CQ, Lv Q, Tang XJ, Li LM, Fang QX, Yu J, Han M, Gao JQ

International Journal of Nanomedicine 2012, 7:4099-4107

Published Date: 30 July 2012

Cross-linked acrylic hydrogel for the controlled delivery of hydrophobic drugs in cancer therapy

Deepa G, Thulasidasan AK, Anto RJ, Pillai JJ, Kumar GS

International Journal of Nanomedicine 2012, 7:4077-4088

Published Date: 27 July 2012

Lysine-functionalized nanodiamonds: synthesis, physiochemical characterization, and nucleic acid binding studies

Kaur R, Chitanda JM, Michel D, Maley J, Borondics F, Yang P, Verrall RE, Badea I

International Journal of Nanomedicine 2012, 7:3851-3866

Published Date: 19 July 2012

Study on antibacterial alginate-stabilized copper nanoparticles by FT-IR and 2D-IR correlation spectroscopy

Díaz-Visurraga J, Daza C, Pozo C, Becerra A, von Plessing C, García A

International Journal of Nanomedicine 2012, 7:3597-3612

Published Date: 11 July 2012

Brain-targeting study of stearic acid–grafted chitosan micelle drug-delivery system

Xie YT, Du YZ, Yuan H, Hu FQ

International Journal of Nanomedicine 2012, 7:3235-3244

Published Date: 29 June 2012

Localized vitreous adhesion to the retina after ocular contusion with a baseball

Kimura M, Nishimura A, Sugiyama K

Clinical Ophthalmology 2012, 6:879-884

Published Date: 8 June 2012

In vitro cellular uptake of evodiamine and rutaecarpine using a microemulsion

Zhang YT, Huang ZB, Zhang SJ, Zhao JH, Wang Z, Liu Y, Feng NP

International Journal of Nanomedicine 2012, 7:2465-2472

Published Date: 18 May 2012

Theranostic liposomes loaded with quantum dots and apomorphine for brain targeting and bioimaging

Wen CJ, Zhang LW, Al-Suwayeh SA, Yen TC, Fang JY

International Journal of Nanomedicine 2012, 7:1599-1611

Published Date: 26 March 2012