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Simple filter microchip for rapid separation of plasma and viruses from whole blood

Authors Wang SQ, Sarenac D, Chen MH, Huang SH, Giguel FF, Kuritzkes DR, Demirci U

Published Date September 2012 Volume 2012:7 Pages 5019—5028

DOI http://dx.doi.org/10.2147/IJN.S32579

Received 3 April 2012, Accepted 5 July 2012, Published 17 September 2012

ShuQi Wang,1 Dusan Sarenac,1 Michael H Chen,1 Shih-Han Huang,1 Francoise F Giguel,2 Daniel R Kuritzkes,3 Utkan Demirci1,4

1
Bio-acoustic MEMS in Medicine Laboratory, Department of Medicine, Division of Biomedical Engineering, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA; 2Infectious Diseases Unit, Massachusetts General Hospital, Boston, MA, USA; 3Section of Retroviral Therapeutics, Brigham and Women's Hospital, Boston, MA, USA; 4Harvard-MIT Health Sciences and Technology, Cambridge, MA, USA

Abstract: Sample preparation is a significant challenge for detection and sensing technologies, since the presence of blood cells can interfere with the accuracy and reliability of virus detection at the nanoscale for point-of-care testing. To the best of our knowledge, there is not an existing on-chip virus isolation technology that does not use complex fluidic pumps. Here, we presented a lab-on-a-chip filter device to isolate plasma and viruses from unprocessed whole blood based on size exclusion without using a micropump. We demonstrated that viruses (eg, HIV) can be separated on a filter-based chip (2-µm pore size) from HIV-spiked whole blood at high recovery efficiencies of 89.9% ± 5.0%, 80.5% ± 4.3%, and 78.2% ± 3.8%, for viral loads of 1000, 10,000 and 100,000 copies/mL, respectively. Meanwhile, 81.7% ± 6.7% of red blood cells and 89.5% ± 2.4% of white blood cells were retained on 2 µm pore–sized filter microchips. We also tested these filter microchips with seven HIV-infected patient samples and observed recovery efficiencies ranging from 73.1% ± 8.3% to 82.5% ± 4.1%. These results are first steps towards developing disposable point-of-care diagnostics and monitoring devices for resource-constrained settings, as well as hospital and primary care settings.

Keywords: microchip, filtration, virus isolation, plasma separation, point-of-care

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Portable microfluidic chip for detection of Escherichia coli in produce and blood

Wang S, Inci F, Chaunzwa TL, Ramanujam A, Vasudevan A, Subramanian S, Ip AC, Sridharan B, Gurkan UA, Demirci U

International Journal of Nanomedicine 2012, 7:2591-2600

Published Date: 29 May 2012

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