Role of ICAM-1 and E-selectin gene polymorphisms in pathogenesis of PAOD in Egyptian patients

Original Research

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Authors: Olfat Shaker, Amr Zahra, Ahmed Sayed, et al

Published Date December 2009 Volume 2010:6 Pages 9 - 15

DOI: http://dx.doi.org/10.2147/VHRM.S8143

Olfat Shaker¹, Amr Zahra², Ahmed Sayed³, Ayman Refaat⁴, Zakaria El-Khaiat⁵, Gehan Hegazy⁵, Khaled El-Hindawi³, Mohamed Ay-El Deen³

¹Department of Medical Biochemistry, ³Vascular Surgery, Faculty of Medicine, Cairo University, Cairo, Egypt; ²Department of Medical Biochemistry, Faculty of Medicine, Fayoum University, Al Fayyum, Egypt; ⁴Vasular Surgery, Faculty of Medicine, Beni Suef University, Beni-Suef, Egypt; ⁵Medical Biochemistry Department, National Research Center, Cairo, Egypt

Background: Intercellular adhesion molecule-1 (ICAM-1) and E-selectin have been shown to predict cardiovascular disease (CVD) such as myocardial infarction, stroke, and peripheral arterial occlusive disease (PAOD).

Methods: Two mutations, S128R in E-selectin and K469E in ICAM-1, were investigated in 156 patients with PAOD and 100 control subjects using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis in an Egyptian population.

Results: The distribution of E-selectin genotypes in patients affected by PAOD was 84.6% for the AA genotype and 15.4% for the AC genotype. In the control arm the distribution was 97% for the AA genotype and 3% for the AC genotype. There was a statistically significance difference in the distribution of the AC genotype in PAOD patients when compared with the control subjects. Additionally, the distribution of ICAM-1 genotypes in patients affected by PAOD was 30.8% with the EE, 48% with the EK, and 21.2% with the KK genotypes. The distribution of ICAM-1 genotypes in control subjects was 13% EE, 33% EK and 54% KK. The EE genotype was significantly more common in PAOD patients than in the controls.

Conclusion: S128R and K469E polymorphisms were associated with increased risk in PAOD. Early detection of these polymorphic genes helps in early prophylaxis against PAOD.

Keywords: polymorphism, PAOD, E-selectin, ICAM-1, RFLP, genotyping