Cell Health and Cytoskeleton
Open access peer-reviewed scientific and medical journals.
Dove Medical Press is now a member of the Open Access Initiative
An Author's Guide
A guide to help authors get their paper published.
Support Open Access and Dove Press
Promotional Article Monitoring - further details
Favored Author Program
Real benefits for authors, including fast-track processing of papers.
Precisely delivered nanomechanical forces induce blebbing in undifferentiated mouse embryonic stem cells
(4869) Total Article Views
Authors: Alexandra L Hemsley, Diana Hernandez, Christopher Mason, et al
Published Date January 2011
Volume 2011:3 Pages 23 - 34
Alexandra L Hemsley1, Diana Hernandez1, Christopher Mason1, Andrew E Pelling2,3, Farlan S Veraitch1
1Advanced Centre for Biochemical Engineering, 2The London Centre for Nanotechnology, Centre for Nanomedicine, University College London, London, UK; 3Department of Physics, University of Ottawa, Ottawa, ON, Canada
Abstract: The aim of this study was to probe the morphological response of single mouse embryonic stem cells (mESC) to precisely delivered nanomechanical forces. Plating mESC as single cells gave rise to either round compact or flattened fibroblastic morphologies. The expression of OCT4 and Nanog was reduced in flattened cells, indicating that this population had begun to differentiate. Upon application of >5 nN of force, using atomic force microscopy and simultaneous laser scanning confocal microscopy, round cells, but not flattened cells, were capable of forming mechanically induced blebs (miBlebs). Flattened cells appeared to have a more highly developed cytoskeleton than undifferentiated stem cells as characterized by the distribution of phospho-ezrin-radixin-moesin (pERM). Higher levels of pERM and an inability to form miBlebs in flattened cells imply that the earliest stages of embryonic stem cell differentiation are associated with the development of stronger mechanical links between the plasma membrane and the cytoskeleton.
Keywords: blebbing, embryonic stem cells, atomic force microscopy, cytoskeleton development, differentiation
Cannotea Citeulike Del.icio.us Facebook LinkedIn Twitter
Readers of this article also read:
"You do a tremendous job!!" Ruben Restrepo, University of Texas Health Science Center, San Antonio.
- Evolution of a domain conserved in microtubule-associated proteins of eukaryotes
- Is gene activity in plant cells affected by UMTS-irradiation? A whole genome approach
- Overview of the LDL receptor: relevance to cholesterol metabolism and future approaches for the treatment of coronary heart disease
- Discrimination between biological interfaces and crystal-packing contacts