Osteogenic capacity of nanocrystalline bone cement in a weight-bearing defect at the ovine tibial metaphysis

Christoph Harms,¹ Kai Helms,¹ Tibor Taschner,¹ Ioannis Stratos,¹ Anita Ignatius,⁵ Thomas Gerber,² Solvig Lenz,³ Stefan Rammelt,⁶ Brigitte Vollmar,⁴ Thomas Mittlmeier^1
1Department of Trauma and Reconstructive Surgery, ²Department for Materials Research and Nanostructures, Institute for Physics, ³Department of Oral and Maxillofacial Plastic Surgery, ⁴Institute for Experimental Surgery, University of Rostock, Rostock, ⁵Institute of Orthopaedic Research and Biomechanics, University of Ulm, Ulm, ⁶Clinic of Trauma and Reconstructive Surgery, University Hospital "Carl Gustav Carus", Dresden, Germany

Abstract: The synthetic material Nanobone® (hydroxyapatite nanocrystallines embedded in a porous silica gel matrix) was examined in vivo using a standardized bone defect model in the ovine tibial metaphysis. A standardized 6 × 12 × 24-mm bone defect was created below the articular surface of the medial tibia condyles on both hind legs of 18 adult sheep. The defect on the right side was filled with Nanobone®, while the defect on the contralateral side was left empty. The tibial heads of six sheep were analyzed after 6, 12, and 26 weeks each. The histological and radiological analysis of the defect on the control side did not reveal any bone formation after the total of 26 weeks. In contrast, the microcomputed tomography analysis of the defect filled with Nanobone® showed a 55%, 72%, and 74% volume fraction of structures with bone density after 6, 12, and 26 weeks, respectively. Quantitative histomorphological analysis after 6, and 12 weeks revealed an osteoneogenesis of 22%, and 36%, respectively. Hematoxylin and eosin sections demonstrated multinucleated giant cells on the surface of the biomaterial and resorption lacunae, indicating osteoclastic resorptive activity. Nanobone® appears to be a highly potent bone substitute material with osteoconductive properties in a loaded large animal defect model, supporting the potential use of Nanobone® also in humans.

Keywords: biocompatibility, bone ingrowth, hydroxyapatite, osseointegration, osteoconduction