skip to content
Dovepress - Open Access to Scientific and Medical Research
View our mobile site

8852

Non-invasive and transdermal measurement of blood uric acid level in human by electroporation and reverse iontophoresis

Original Research

(1653) Views  (372) Full article downloads

Authors: Chih-Kuei Lee, Congo Tak-Shing Ching, Tai-Ping Sun, et al

Published Date November 2010 Volume 2010:5 Pages 991 - 997
DOI: http://dx.doi.org/10.2147/IJN.S14284

Chih-Kuei Lee1, Congo Tak-Shing Ching2–4, Tai-Ping Sun2,3, Chun-Lang Tsai2, Wei Huang5, Hsin-Hung Huang6, Jen-Fu Kuo7, Li-Hang Lai6, Mei-Ya Chien8, Hsin-Hui Tseng9, Hui-Tzu Pan10, Shiow-Yuan Huang4, Hsiu-Li Shieh3, Wei-Hao Liu2, Chia-Ming Liu2, Hsin-Wei Huang2
1Hopkins Health Management Center, Puli Christian Hospital, Nantou, Taiwan, Republic of China; 2Graduate Institute of Biomedicine and Biomedical Technology, National Chi Nan University, Nantou, Taiwan, Republic of China; 3Department of Electrical Engineering, National Chi Nan University, Nantou, Taiwan, Republic of China; 4Department of Photonics and Communication Engineering, Asia University, Taichung, Taiwan, Republic of China; 5Division of Immunology and Rheumatology, Puli Christian Hospital, Nantou, Taiwan, Republic of China; 6Family Medical Department, Puli Christian Hospital, Nantou, Taiwan, Republic of China; 7Division of Endocrinology and Metabolism, Puli Christian Hospital, Nantou, Taiwan, Republic of China; 8Nutrition Department, Puli Christian Hospital, Nantou, Taiwan, Republic of China; 9Aboriginal Health Care, Puli Christian Hospital, Nantou, Taiwan, Republic of China; 10Department of Medical Research and Education, Puli Christian Hospital, Nantou, Taiwan, Republic of China

Abstract: The aim of this study was to find out the optimum combination of electroporation (EP) and reverse iontophoresis (RI) on noninvasive and transdermal determination of blood uric acid level in humans. EP is the use of high-voltage electric pulse to create nano-channels on the stratum corneum, temporarily and reversibly. RI is the use of small current to facilitate both charged and uncharged molecule transportation across the skin. It is believed that the combination of these two techniques has additional benefits on the molecules’ extraction across the human skin. In vitro studies using porcine skin and diffusion cell have indicated that the optimum mode for transdermal uric acid extraction is the combination of RI with symmetrical biphasic direct current (current density = 0.3 mA/cm2; phase duration = 180 s) and EP with 10 pulses per second (voltage = 100 V/cm2; pulse width = 1 ms). This optimum mode was applied to six human subjects. Uric acid was successfully extracted through the subjects’ skin into the collection solution. A good correlation (r2 = 0.88) between the subject’s blood uric acid level and uric acid concentrations in collection solutions was observed. The results suggest that it may be possible to noninvasively and transdermally determine blood uric acid levels.

Keywords: Reverse iontophoresis, electroporation, uric acid, monitoring, noninvasive, transdermal




 

Other articles by Dr Congo Tak-Sing Ching



Readers of this article also read:

Evidence-based decision-making within the context of globalization: A “Why–What–How” for leaders and managers of health care organizations
Radiolucency below the crown of mandibular horizontal incompletely impacted third molars and acute inflammation in men with diabetes
"Globalized public health.” A transdisciplinary comprehensive framework for analyzing contemporary globalization’s influences on the field of public health
The pathophysiology of bronchiectasis
A preliminary study of the use of bioimpedance in the screening of squamous tongue cancer
Gout disease-specific quality of life and the association with gout characteristics
Antibacterial properties of silver nanoparticles in three different sizes and their nanocomposites with a new waterborne polyurethane
Amino acid management of Parkinson’s disease: a case study
Comparison of two treatments for coxarthrosis: local hyperthermia versus radio electric asymmetrical brain stimulation
Radio electric asymmetric brain stimulation in the treatment of behavioral and psychiatric symptoms in Alzheimer disease