skip to content
Dovepress - Open Access to Scientific and Medical Research
View our mobile site

8852

No evidence for activation of TH1 or TH17 pathways in unstimulated peripheral blood mononuclear cells from children with β-cell autoimmunity or T1D

Original Research

(3186) Views  (740) Full article downloads

Authors: Jenny Walldén, Jarno Honkanen, Jorma Ilonen, Johnny Ludvigsson, Outi Vaarala

Published Date September 2008 Volume 2008:1 Pages 11 - 17
DOI: http://dx.doi.org/10.2147/JIR.S3547

Jenny Walldén1, Jarno Honkanen2, Jorma Ilonen3, Johnny Ludvigsson1, Outi Vaarala2

1Linköping University, Faculty of Health Sciences, Department of Clinical and Experimental Medicine, Division of Pediatrics and Diabetes Research Center, Linköping, Sweden; 2Department of Viral Diseases and Immunology, Laboratory for Immunobiology, National Public Health Institute, Helsinki, Finland; 3Department of Clinical Microbiology, University of Kuopio, Kuopio and Immunogenetics Laboratory, University of Turku, Turku, Finland

Introduction: The balance between TH1, TH2, TH17, and regulatory T cells has been suggested to be disturbed in type 1 diabetes (T1D). We investigated this balance in peripheral blood mononuclear cells (PBMC) from children at risk of developing T1D and children with T1D.

Methods: We studied PBMC expression levels of markers related to TH1 (T-bet, IL-12Rβ1, IL-12Rβ2), TH2 (GATA-3, IL-4Rα), TH17 (IL-17A), and regulatory T cells (Foxp3, ICOS, and CTLA-4) with real-time polymerase chain reaction from 17 children with T1D, 13 children with β-cell autoimmunity, 15 children with T1D risk-associated human leukocyte antigen (HLA) haplotypes, and 24 healthy, control children.

Results: We observed decreased expression levels of GATA-3 by PBMC of healthy children with autoantibodies compared to healthy, control children (p = 0.014) or children with HLA risk alleles (p = 0.032). Children with T1D demonstrated lower expression levels of T-bet, IL-12Rβ1, and IL-4Rα both at diagnosis and 12 months later.

Conclusion: We found no indication of aberrant activation of TH1, TH17, or Treg in peripheral blood from children with or without risk of T1D. The observed immunological differences between children at risk of and with T1D should be considered when immunopathogenesis of β-cell destruction is studied.

Keywords: transcription factor, type 1 diabetes, mononuclear cells








Readers of this article also read:

Radiolucency below the crown of mandibular horizontal incompletely impacted third molars and acute inflammation in men with diabetes
Berberine: metabolic and cardiovascular effects in preclinical and clinical trials
Critical appraisal of the role of glucosamine and chondroitin in the management of osteoarthritis of the knee
Ego mechanisms of defense are associated with patients’ preference of treatment modality independent of psychological distress in end-stage renal disease
Can a gentamicin-specific chart reduce neonatal medication errors?
Health literacy and health seeking behavior among older men in a middle-income nation
Nephroprotective action of glycosaminoglycans: why the pharmacological properties of sulodexide might be reconsidered
Dashboards in neonatology
Anesthesiologists’ perception of patients’ anxiety under regional anesthesia
Everolimus-eluting stents: update on current clinical studies
  • Testimonials

    "You do a tremendous job!!" Ruben Restrepo, The University of Texas Health Science Center at San Antonio