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Neurite development in PC12 cells cultured on nanopillars and nanopores with sizes comparable with filopodia

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Authors: Furqan Haq, Venkatramani Anandan, Charles Keith, Guigen Zhang

Published Date May 2007 Volume 2007:2(1) Pages 107 - 115
DOI: http://dx.doi.org/10.2147/IJN.S

Furqan Haq1, Venkatramani Anandan1, Charles Keith2,4, Guigen Zhang1,3,4

1Micro/Nano Bioengineering Lab, Department of Biological and Agricultural Engineering; 2Department of Cellular Biology, 3Nanoscale Science and Engineering Center, 4Faculty of Engineering, University of Georgia, Athens, GA 30602, USA

Abstract: We investigated the effect of nanoscale topography on neurite development in pheochromocytoma (PC12 cells) by culturing the cells on substrates having nanoscale pillars and pores with sizes comparable with filipodia. We found that cells on nanopillars and nanopores developed fewer and shorter neurites than cells on smooth substrates, and that cells on nanopores developed more and longer neurites than cells on nanopillars. These results suggest that PC12 cells were spatially aware of the difference in the nanoscale structures of the underlying substrates and responded differently in their neurite extension. This finding points to the possibility of using nanoscale topographic features to control neurite development in neurons.

Keywords: nanopillars, nanopores, substrates, neurite extension, PC12 cells








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