Magnetic nanoparticle of Fe₃O₄ and 5-bromotetrandrin interact synergistically to induce apoptosis by daunorubicin in leukemia cells

Original Research

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Authors: Baoan Chen, Jian Cheng, Mingfang Shen, Feng Gao, Wenlin Xu, et al 

Published Date March 2009 Volume 2009:4 Pages 65 - 71

DOI: http://dx.doi.org/10.2147/IJN.S5036

Baoan Chen¹*, Jian Cheng¹*, Mingfang Shen¹, Feng Gao¹, Wenlin Xu², et al

¹Department of Hematology;²Department of Hematology, The Affiliated People’s Hospital, Jiangsu University, Zhenjiang, P.R China, et al *These authors have contributed equally to this work.

Abstract: Apoptosis is a common pathway that finally mediated the killing functions of anticancer drugs, which is an important cause of multidrug resistance (MDR). The aim of this study was to investigate the potential benefit of combination therapy with magnetic nanoparticle of Fe₃O₄ (MNP(Fe₃O₄)) and 5-bromotetrandrin (BrTet). Analysis of the apoptosis percentage showed that combination of daunorubicin (DNR) with either MNP(Fe₃O₄) or BrTet exerted a potent cytotoxic effect on K562/A02 cells, while MNP(Fe₃O₄) and BrTet cotreatment can synergistically enhance DNR-induced apoptosis. Importantly, we confirmed that the distinct synergism effect of that composite on reverse multidrug resistance may owe to the regulation of various proliferative and antiapoptotic gene products, including P53 and caspase-3. Thus our in vitro data strongly suggests a potential clinical application of MNP(Fe₃O₄) and BrTet combination on CML.

Keywords: K562/A02 leukemic cells, multidrug resistance, magnetic nanoparticle of Fe₃O₄, 5-bromotetrandrine, apoptosis, P53, caspase-3