Back to Browse Journals » International Journal of Nanomedicine » Volume 6

Interaction of an anticancer peptide fragment of azurin with p53 and its isolated domains studied by atomic force spectroscopy

Authors Bizzarri AR, Santini S, Coppari E, Bucciantini M, Di Agostino S, Yamada T, Beattie CW, Cannistraro S

Published Date November 2011 Volume 2011:6 Pages 3011—3019

DOI http://dx.doi.org/10.2147/IJN.S26155

Published 24 November 2011

Anna Rita Bizzarri1, Simona Santini1, Emilia Coppari1, Monica Bucciantini2, Silvia Di Agostino3, Tohru Yamada4, Craig W Beattie4, Salvatore Cannistraro1
1
Biophysics and Nanoscience Centre, CNISM, Facoltà di Scienze, Università della Tuscia, Viterbo, 2Department of Biochemical Sciences, University of Florence, Florence, 3Molecular Oncogenesis Laboratory, Experimental Oncology Department, Regina Elena Cancer Institute, Rome, Italy; 4Department of Surgical Oncology, University of Illinois College of Medicine, Chicago, IL, USA

Abstract: p28 is a 28-amino acid peptide fragment of the cupredoxin azurin derived from Pseudomonas aeruginosa that preferentially penetrates cancerous cells and arrests their proliferation in vitro and in vivo. Its antitumor activity reportedly arises from post-translational stabilization of the tumor suppressor p53 normally downregulated by the binding of several ubiquitin ligases. This would require p28 to specifically bind to p53 to inhibit specific ligases from initiating proteosome-mediated degradation. In this study, atomic force spectroscopy, a nanotechnological approach, was used to investigate the interaction of p28 with full-length p53 and its isolated domains at the single molecule level. Analysis of the unbinding forces and the dissociation rate constant suggest that p28 forms a stable complex with the DNA-binding domain of p53, inhibiting the binding of ubiquitin ligases other than Mdm2 to reduce proteasomal degradation of p53.

Keywords: AFS, cancer physics, unbinding forces

Download Article [PDF] View Full Text [HTML] 

Creative Commons License This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution - Non Commercial (unported, v3.0) License. The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. Permissions beyond the scope of the License are administered by Dove Medical Press Limited. Information on how to request permission may be found at: http://www.dovepress.com/permissions.php

Other article by this author:

SERS-based nanobiosensing for ultrasensitive detection of the p53 tumor suppressor

Domenici F, Bizzarri AR, Cannistraro S

International Journal of Nanomedicine 2011, 6:2033-2042

Published Date: 19 September 2011

Readers of this article also read:

Strategies for improving early detection and diagnosis of neovascular age-related macular degeneration

Keane PA, de Salvo G, Sim DA, Goverdhan S, Agrawal R, Tufail A

Clinical Ophthalmology 2015, 9:353-366

Published Date: 17 February 2015

Increase in the proportion of patients hospitalized with acute myocardial infarction with do-not-resuscitate orders already in place between 2001 and 2007: a nonconcurrent prospective study

Saczynski JS, Gabbay E, McManus DD, McManus R, Gore JM, Gurwitz JH, Lessard D, Goldberg RJ

Clinical Epidemiology 2012, 4:267-274

Published Date: 19 October 2012

Improved biological properties and hypouricemic effects of uricase from Candida utilis loaded in novel alkaline enzymosomes

Tan QY, Zhang JQ, Wang N, Yang H, Li X, Xiong HR, Wu JY, Zhao CJ, Wang H, Yin HF

International Journal of Nanomedicine 2012, 7:3929-3938

Published Date: 23 July 2012

Servant leadership: a case study of a Canadian health care innovator

Vanderpyl TH

Journal of Healthcare Leadership 2012, 4:9-16

Published Date: 16 February 2012

Eudragit nanoparticles containing genistein: formulation, development, and bioavailability assessment

Tang J, Xu N, Ji H, Liu H, Wang Z, Wu L

International Journal of Nanomedicine 2011, 6:2429-2435

Published Date: 19 October 2011

Solvent effects on structural and thermochemical properties of p53 tumor-suppressor gene: a molecular modeling approach in drug design

Irani S, Atyabi SM, Mivehchi H, Siadat SD, Aghasadeghi MR, Farhangi A

International Journal of Nanomedicine 2011, 6:2063-2069

Published Date: 20 September 2011

Non-invasive and transdermal measurement of blood uric acid level in human by electroporation and reverse iontophoresis

Chih-Kuei Lee, Congo Tak-Shing Ching, Tai-Ping Sun, et al

International Journal of Nanomedicine 2010, 5:991-997

Published Date: 19 November 2010

Pharmacokinetics and tolerance study of intravitreal injection of dexamethasone-loaded nanoparticles in rabbits

Linhua Zhang, Yue Li, Chao Zhang, et al

International Journal of Nanomedicine 2009, 4:175-183

Published Date: 4 September 2009