skip to content
Dovepress - Open Access to Scientific and Medical Research
View our mobile site

8852

IgG from patients with systemic sclerosis bind to DNA antitopoisomerase 1 in normal human fibroblasts extracts

Original Research

(2587) Views  (609) Full article downloads

Authors: Mathieu C Tamby, Amélie Servettaz, Nicolas Tamas, Joseph Reinbolt, Frédéric Caux, et al

Published Date September 2008 Volume 2008:2(3) Pages 583 - 591
DOI: http://dx.doi.org/10.2147/BTT.S3188

Mathieu C Tamby1, Amélie Servettaz1, Nicolas Tamas1, Joseph Reinbolt2, Frédéric Caux3, Olivier Meyer4, Yannick Allanore5, André Kahan5, Loïc Guillevin6, Luc Mouthon1,6

1Paris-Descartes University, Faculty of Medicine, UPRES-EA 4058, Paris, France; 2UPR 9002, Centre National de la Recherche Scientifique, Strasbourg, France; 3UPRES EA 2436, Paris-Nord University, Bobigny, France; 4Rheumatology Department, Bichat Hospital, Assistance Publique-Hôpitaux de Paris (AP-HP), Paris, France; 5Rheumatology A Department, Cochin Hospital, AP-HP; 6Paris-Descartes University, Faculty of Medicine, Department of Internal Medicine and French Reference Center for Necrotizing Vasculitides and Systemic Sclerosis, Cochin Hospital, AP-HP, Paris, France

Abstract: By using a semi-quantitative immunoblotting technique, we have analyzed serum immunoglobulin G (IgG) reactivities of patients with limited cutaneous systemic sclerosis and anticentromere antibodies, patients with diffuse systemic sclerosis and antitopoisomerase 1 antibodies, patients with diffuse systemic sclerosis without antitopoisomerase 1 or anticentromere antibodies and age- and gender-matched healthy controls with normal human skin fibroblasts and HEp-2 cells antigens. Serum IgG reactivities of patients with diffuse systemic sclerosis and antitopoisomerase 1 antibodies differed significantly from those of healthy controls or systemic sclerosis patients in other groups for reactivity with fibroblast proteins. IgG from patients with antitopoisomerase 1 antibodies bound to a 90 kDa fibroblast band and to a 100 kDa protein band in a HEp-2 cell protein extract. These two bands were further identified as DNA topoisomerase 1. Our results indicate that IgG from patients with diffuse systemic sclerosis bind DNA topoisomerase 1 in normal human fibroblasts extracts.

Keywords: systemic sclerosis, autoantibodies, IgG, fibroblast, DNA topoisomerase 1








Readers of this article also read:

α-L-iduronidase therapy for mucopolysaccharidosis type I
Prevalence of risk factors, coronary and systemic atherosclerosis in abdominal aortic aneurysm: Comparison with high cardiovascular risk population
Review of recombinant human deoxyribonuclease (rhDNase) in the management of patients with cystic fibrosis
Long term management of patients with cryopyrin-associated periodic syndromes (CAPS): focus on rilonacept (IL-1 Trap)
Therapeutic vaccines for malignant brain tumors
Gene targeted therapeutics for liver disease in alpha-1 antitrypsin deficiency
Abatacept in difficult-to-treat juvenile idiopathic arthritis
Retinal nerve fiber layer evaluation in multiple sclerosis with spectral domain optical coherence tomography
Seroprevalence of Toxoplasma gondii IgG antibody in HIV-infected patients at the Lagos University Teaching Hospital
Primary care for diabetes mellitus: perspective from older patients
  • Testimonials

    "... I was impressed at the rapidity of publication from submission to final acceptance." Dr Edwin Thrower, PhD, Yale University

  • Journal Indexing

    See where all the Dove Press journals are indexed