Back to Journals » International Journal of Nanomedicine » Volume 7

Electrochemical impedimetric biosensor based on a nanostructured polycarbonate substrate

Authors Chen, Wu C, Tsai J , Gou-Jen Wang G 

Received 10 October 2011

Accepted for publication 30 November 2011

Published 6 January 2012 Volume 2012:7 Pages 133—140

DOI https://doi.org/10.2147/IJN.S27225

Review by Single anonymous peer review

Peer reviewer comments 2



Yu-Shan Chen1, Chia-Che Wu1, Jaw-Ji Tsai2, Gou-Jen Wang1,3
1Department of Mechanical Engineering, National Chung-Hsing University, 2Department of Medical Education and Research, Taichung Veterans General Hospital, 3Graduate Institute of Biomedical Engineering, National Chung-Hsing University, Taichung, Taiwan

Abstract: This study integrates the techniques of nanoelectroforming, hot-embossing, and electrochemical deposition to develop a disposable, low-cost, and high sensitivity nanostructure biosensor. A modified anodic aluminum oxide barrier-layer surface was used as the template for thin nickel film deposition. After etching the anodic aluminum oxide template off, a three-dimensional mold of the concave nanostructure array was created. The fabricated three-dimensional nickel mold was further used for replica molding of a nanostructure polycarbonate substrate by hot-embossing. A thin gold film was then sputtered onto the polycarbonate substrate to form the electrode, followed by deposition of an orderly and uniform gold nanoparticle layer on the three-dimensional gold electrode using electrochemical deposition. Finally, silver nanoparticles were deposited on the uniformly deposited gold nanoparticles to enhance the conductivity of the sensor. Electrochemical impedance spectroscopy analysis was then used to detect the concentration of the target element. The sensitivity of the proposed scheme on the detection of the dust mite antigen, Der p2, reached 0.1 pg/mL.

Keywords: nanoelectroforming, nanostructure polycarbonate substrate, gold nanoparticles, silver nanoparticles, electrochemical impedance spectroscopy

Creative Commons License © 2012 The Author(s). This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution - Non Commercial (unported, v3.0) License. By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms.