International Journal of Nanomedicine
Open access peer-reviewed scientific and medical journals.
Dove Medical Press is now a member of the Open Access Initiative
An Author's Guide
A guide to help authors get their paper published.
Support Open Access and Dove Press
Promotional Article Monitoring - further details
Favored Author Program
Real benefits for authors, including fast-track processing of papers.
Increased nanoparticle penetration in collagenase-treated multicellular spheroids
(6017) Total Article Views
Authors: Thomas T Goodman, Peggy L Olive, Suzie H Pun
Published Date August 2007
Volume 2007:2(2) Pages 265 - 274
Thomas T Goodman1, Peggy L Olive2, Suzie H Pun1
1Department of Bioengineering, University of Washington, Seattle, WA, USA; 2BC Cancer Research Centre, Vancouver, BC, Canada
Abstract: The extracellular matrix of solid tumors presents a transport barrier that restricts nanoparticle penetration, thereby limiting the efficacy of nano-sized delivery vehicles for cancer imaging and therapy. In this study, the effect of nanoparticle size and collagenase treatment on penetration of carboxylated polystyrene nanoparticles was systematically assessed in a multicellular spheroid model. Penetration of the nanoparticles into the spheroid core was limited to particles smaller than 100 nm. Collagenase treatment of spheroids resulted in significantly increased penetration of nanoparticles up to 100 nm with only a minor increase in particle penetration observed for particles larger than 100 nm. Collagenase was immobilized onto the surface of nanoparticles for site-specific degradation of ECM proteins. Collagenase-coated, 100 nm nanoparticles demonstrated a 4-fold increase in the number of particles delivered to the spheroid core compared with control nanoparticles. Thus, nanoparticle delivery to solid tumors may be substantially improved by the incorporation of ECM-modulating enzymes in the delivery formulation.
Keywords: nanoparticles, collagenase, cancer treatment, spheroids, solid tumors
Cannotea Citeulike Del.icio.us Facebook LinkedIn Twitter
Readers of this article also read:
- ITNANO 2013
Join us at the ITNANO Conference in Boston, July 26th - 28th 2013, sponsored by Northeastern University and the International Journal of Nanomedicine.
- Impact Factors
- Have an opinion about one of our articles?
We encourage you to write a letter to the editor.
- Interested in being a peer-reviewer?
Click here to register.
Authors are welcome to send an abstract or draft manuscript to obtain a view from the Editor about the suitability of their paper. Please email here and include which journal you are interested in submitting your manuscript to. Our Editors will do a quick review of your paper and advise if they believe it is appropriate for submission to their journal.
- Applications of gold nanoparticles in cancer nanotechnology
- Fungus-mediated biological synthesis of gold nanoparticles: potential in detection of liver cancer
- Short communication: carboxylate functionalized superparamagnetic iron oxide nanoparticles (SPION) for the reduction of S. aureus growth post biofilm formation
- Formulation and evaluation of drug-loaded targeted magnetic microspheres for cancer therapy