Back to Browse Journals » International Journal of Nanomedicine » Volume 2 » Issue 2

Increased nanoparticle penetration in collagenase-treated multicellular spheroids

Authors Thomas T Goodman, Peggy L Olive, Suzie H Pun

Published Date August 2007 Volume 2007:2(2) Pages 265—274

DOI

Published 16 August 2007

Thomas T Goodman1, Peggy L Olive2, Suzie H Pun1

1Department of Bioengineering, University of Washington, Seattle, WA, USA; 2BC Cancer Research Centre, Vancouver, BC, Canada

Abstract: The extracellular matrix of solid tumors presents a transport barrier that restricts nanoparticle penetration, thereby limiting the efficacy of nano-sized delivery vehicles for cancer imaging and therapy. In this study, the effect of nanoparticle size and collagenase treatment on penetration of carboxylated polystyrene nanoparticles was systematically assessed in a multicellular spheroid model. Penetration of the nanoparticles into the spheroid core was limited to particles smaller than 100 nm. Collagenase treatment of spheroids resulted in significantly increased penetration of nanoparticles up to 100 nm with only a minor increase in particle penetration observed for particles larger than 100 nm. Collagenase was immobilized onto the surface of nanoparticles for site-specific degradation of ECM proteins. Collagenase-coated, 100 nm nanoparticles demonstrated a 4-fold increase in the number of particles delivered to the spheroid core compared with control nanoparticles. Thus, nanoparticle delivery to solid tumors may be substantially improved by the incorporation of ECM-modulating enzymes in the delivery formulation.

Keywords: nanoparticles, collagenase, cancer treatment, spheroids, solid tumors

Download Article [PDF] 

Readers of this article also read:

Preparation and characterization of flexible nanoliposomes loaded with daptomycin, a novel antibiotic, for topical skin therapy

Li C, Zhang X, Huang X, Wang X, Liao G, Chen Z

International Journal of Nanomedicine 2013, 8:1285-1292

Published Date: 24 March 2013

Long-term MRI tracking of dual-labeled adipose-derived stem cells homing into mouse carotid artery injury

Qin JB, Li KA, Li XX, Xie QS, Lin JY, Ye KC, Jiang ME, Zhang GX, Lu XW

International Journal of Nanomedicine 2012, 7:5191-5203

Published Date: 2 October 2012

Erratum

Marusza W, Mlynarczyk G, Olszanski R, Netsvyetayeva I, Obrowski M, Iannitti T, Palmieri B

International Journal of Nanomedicine 2012, 7:4119-4120

Published Date: 27 July 2012

Enhanced bioavailability and efficiency of curcumin for the treatment of asthma by its formulation in solid lipid nanoparticles

Wang W, Zhu R, Xie Q, Li A, Xiao Y, Liu H, Cui D, Chen Y, Wang S

International Journal of Nanomedicine 2012, 7:3667-3677

Published Date: 17 July 2012

Highly efficient magnetic targeting of mesenchymal stem cells in spinal cord injury

Vaněček V, Zablotskii V, Forostyak S, Růžička J, Herynek V, Babič M, Jendelová P, Kubinová Š, Dejneka A, Syková E

International Journal of Nanomedicine 2012, 7:3719-3730

Published Date: 16 July 2012

Use of clofarabine for acute childhood leukemia

A Pession, R Masetti, K Kleinschmidt, et al

Biologics: Targets and Therapy 2010, 4:111-118

Published Date: 24 June 2010

Role of aliskiren in cardio-renal protection and use in hypertensives with multiple risk factors

Eduardo Pimenta, Suzanne Oparil

Vascular Health and Risk Management 2009, 5:453-463

Published Date: 19 May 2009