A new Vitreoscilla filiformis extract grown on spa water-enriched medium activates endogenous cutaneous antioxidant and antimicrobial defenses through a potential Toll-like receptor 2/protein kinase C, zeta transduction pathway

Yann F Mahe,¹ Marie-Jesus Perez,¹ Charlotte Tacheau,¹ Chantal Fanchon,² Richard Martin,³ Françoise Rousset,¹ Sophie Seite<sup>4

1</sup>L’Oreal Research and Innovation, Clichy, ²L’Oréal Research and Innovation, Chevilly Larue, ³L’Oréal Research and Innovation Tours, ⁴La Roche-Posay Pharmaceutical Laboratories, Asnières, France

Abstract: Vitreoscilla filiformis (VF) biomass (VFB) has been widely used in cosmetic preparations and shown to modulate the major inducible free-radical scavenger mitochondrial superoxide dismutase in skin cells. By adding La Roche-Posay (LRP) thermal spring water to the VF culture medium, we obtained a biomass (LRP-VFB) with a similar mitochondrial superoxide dismutase activation capacity to VF. Also, the new biomass more powerfully stimulated mRNA expression and antimicrobial peptides in reconstructed epidermis. Interestingly, a predictive computer model that analyzed transducing events within skin epidermal cells suggested that this protective activity may involve the Toll-like receptor 2/protein kinase C, zeta transduction pathway. Protein kinase C, zeta inhibition was effectively shown to abolish VFB-induced gene stimulation and confirmed this hypothesis. This thus opens new avenues for investigation into the improvement of skin homeostatic defense in relation to the control of its physiological microbiota and innate immunity.

Keywords: innate skin defenses, TLR2, PKCz, La Roche-Posay, mitochondrial superoxide dismutase, SOD2