skip to content
Dovepress - Open Access to Scientific and Medical Research
View our mobile site

8830

A comparative study of DNA damage in patients suffering from diabetes and thyroid dysfunction and complications

Original Research

(1907) Views  (467) Full article downloads

Authors: Nima V Thakkar, Sunita M Jain

Published Date September 2010 Volume 2010:2 Pages 199 - 205
DOI: http://dx.doi.org/10.2147/CPAA.S11366

Nima V Thakkar, Sunita M Jain
Department of Pharmacology, L.M. College of Pharmacy, Ahmedabad, Gujarat, India

Objective: The apoptotic DNA levels in blood leukocytes of patients with type 2 diabetes (T2D) and thyroid dysfunction were evaluated.
Materials and methods: Single-cell gel electrophoresis (comet assay) detects migration of DNA from individual cell nuclei following alkaline treatment. Comet assay pattern was studied in individuals with T2D, hypothyroid (HT), hyperthyroid (HeT), and patients suffering from both diabetes mellitus and HT (HT + DM). Results were compared with the normal subjects (n = 9 in each group). The percentage apoptotic cells were calculated from the tail length.
Results: T2D patients showed 92.24% of cell damage compared to HT or HeT patients (51.04% or 54.64%, respectively). Further, increase in cell damage was also observed in HT + DM subjects (P < 0.05). Pharmacologic therapy significantly influenced cell damage. However, age and duration of disease did not show any definite influence on apoptosis.
Conclusion: Dependence of disease seems to be the major contributor of the cell damage. However, thyroid dysfunction did not show any deleterious effects on individual cells under the study.

Keywords: comet assay, type 2 diabetes, hypothyroid, apoptosis

 








Readers of this article also read:

Is gene activity in plant cells affected by UMTS-irradiation? A whole genome approach
Wine consumers’ environmental knowledge and attitudes: Influence on willingness to purchase
Age-related disruption of the lamina dura: Evidence in the mandibular horizontal incompletely impacted third molar
Management of hypertension with fixed dose combinations of candesartan cilexetil and hydrochlorothiazide: patient perspectives and clinical utility
Clinicoradiological features of recurrent ischemic stroke: healthcare for poststroke patients
Mitochondrial therapy for Parkinson’s disease: Neuroprotective pharmaconutrition may be disease-modifying
Analgesic efficacy of oral gabapentin added to standard epidural corticosteroids in patients with failed back surgery
Wine fraud
Using systematically observed clinical encounters (SOCEs) to assess medical students’ skills in clinical settings
Examining human rights and mental health among women in drug abuse treatment centers in Afghanistan
  • Testimonials

    "... I was impressed at the rapidity of publication from submission to final acceptance." Dr Edwin Thrower, PhD, Yale University